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KMID : 0385219990090010031
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Korean Journal of Gerontology
1999 Volume.9 No. 1 p.31 ~ p.35
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Activation of Inducible Nitric Oxide Synthase by Interferon-γ Plus Tumor Necrosis Factor-α in Sertoli Cells
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Kim Hyung-Min
Yoon Wook-Hee
Jin Gyung-Ho
Cha Kwang-Ho
Lee Ki-Nam
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Abstract
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To study the nitric oxide(NO) synthesis and its signalling mechanism in TM4 Sertoli cells, the cells were treated with recombinant tumor necrosis factor-α (rTNF-α), recombinant interleukin-1 α (rIL-1α), or lipopolysaccharide(LPS), either alone or in combination with recombinant interferon-γ (rIFN-γ), and NO synthesis was measured by using the Griess method. TM4 Sertoli cells produced a small amount of NO with the treatment of rIFN-γ. The effect of rIFN-γwas drastically increased by cotreatment with rTNF-γ in a dose-dependent manner, However, combinations of rIL-lα or LPS with rIFN-γ did not synergies to activate cells. RIFN-γ in combination with rTNF-α showed marked increase of the expression of iNOS protein. Protein kinase C inhibitors, staurosporin and polymyxin 5, did not inhibit the synthesis of NO induced by rIFN-γ plus rTNF-α These results suggest that the role of TNF-α is to provide TM4 Sertoli cells with the active cofactor for NO synthesis and TNF-α-induced signaling for induction of NO synthesis is not dependent on protein kinase C activation.
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KEYWORD
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Nitric oxide, TM4 sertoli cells, Tumor necrosis factor-α, Interferon-γ, Protein kinase C
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